Journal: Frontiers in Cardiovascular Medicine

Article Title: Hematopoietic Cell-Specific SLC37A2 Deficiency Accelerates Atherosclerosis in LDL Receptor-Deficient Mice

doi: 10.3389/fcvm.2021.777098

Figure Lengend Snippet: SLC37A2 deficiency promotes oxidized LDL (OxLDL)-induced macrophage inflammation in vitro . (A) SLC37A2 protein expression analyzed by Western blotting in WT and Slc37a2 −/− bone marrow-derived macrophages (BMDMs) treated with 50 μg/ml oxLDL for 0-24 h. (B) Quantification of cellar total cholesterol (TC), free cholesterol (FC), and cholesterol ester (CE) in WT and Slc37a2 −/− BMDMs after treated with or without 25 or 50 μg/ml oxLDL for 24 h. (C) Relative transcript level of cytokines in WT and Slc37a2 −/− BMDMs stimulated with 50 μg/ml oxLDL for 0-24 h, measured by qPCR. Data are representative of two independent experiments with three samples per group (mean ± SEM). * p < 0.05; unpaired, two-tailed Student's t -test.

Article Snippet: To induce foam cell formation, BMDMs were treated with 25 or 50 μg/ml oxLDL (Athens Research & Technology) for 0-24 h. In some experiments, BMDMs were pretreated for 30 min with hexokinase inhibitor 2-deoxy-D-glucose (2-DG; 10 mM, Sigma-Aldrich), actin polymerization inhibitor cytochalasin D (10 μM, Cayman), or fatty acid β-oxidation inhibitor etomoxir (50 μM, Cayman) and subsequently treated with apoptotic cells for an additional 4 h in the presence of each inhibitor.

Techniques: In Vitro, Expressing, Western Blot, Derivative Assay, Two Tailed Test

Journal: International journal of medical sciences

Article Title: P66Shc is increased in peripheral blood mononuclear cells of the patients with obstructive sleep apnea.

doi: 10.7150/ijms.80343

Figure Lengend Snippet: Figure 2. P66Shc mRNA levels (n=73) were positively correlated with (A) plasma 3-NT, (B) oxLDL, and (C) AOPP levels by Spearman’s rank correlation coefficient.

Article Snippet: Enzyme-linked Immunosorbent Assay The level of plasma 3-NT (Elabscience, Catalog#E-EL-0040c, China), oxLDL (Elabscience, Catalog#E-EL-H6021, China) and AOPP (Abbkine, Catalog#KTB1060, China) were detected with the enzyme-linked immunosorbent assay (ELISA) commercial kits according to the recommendations of the manufacturers.

Techniques: Clinical Proteomics

Journal: Drug Design, Development and Therapy

Article Title: Baolier Capsule’s Secret Weapon: Piperine Boosts Cholesterol Excretion to Combat Atherosclerosis

doi: 10.2147/DDDT.S499598

Figure Lengend Snippet: Key Resources Table

Article Snippet: LDL-C assay kit , Elabscience , E-BC-K205-M.

Techniques: Control, shRNA, Sequencing, Staining, Fluorescence, Microscopy, AST Assay, Phospholipid Assay, Mass Spectrometry

Journal: bioRxiv

Article Title: Mast cell-driven adipocyte lipolysis promotes post-expansive VAT atrophy and ectopic hepatic steatosis

doi: 10.64898/2026.01.07.698076

Figure Lengend Snippet: ( a ) Heatmap of MC mediator-related gene expression in EAT in WT mice at week 0 and at week 10 of HFD feeding. n=3/ea. ( b ) Real-time PCR analysis of MC mediator-related genes Mcpt4 (encodes mouse mast cell protease 4 (mMCP-4), Cma1 (encodes mMCP-5), Hdc (encodes histidine decarboxylase), Ext1 (encodes exostosin-1), Ext2 , Ndst1 (encodes N-deacetylase/N-sulphotransferase 1), Ndst2 , Tph1 (encodes tryptophan hydroxylase 1), and Hpgds (encodes hematopoietic prostaglandin D synthase) in EAT in WT mice at week 0, 10, 20 of HFD feeding. n=6-8/ea. ( c/d ) Serum ( c ) and EAT ( d ) 5-HT levels in HFD-fed WT mice. n=6-8/ea. ( e ) EAT 5-HT levels in WT mice received i.p. injections of saline or DSCG from week 0 to 20 of HFD feeding. n=6-8/ea. ( f ) Real-time PCR analysis of Tph1 mRNA levels in adipocytes, SVFs, MC-depleted SVFs, and MCs from WT EAT in HFD-fed mice for 16 weeks. n=5-8/ea. ( g ) 5-HT + (red) and CD117 + (green), 5-HT + (red) and FceRI + (green) immunofluorescent double staining in WT EAT in HFD-fed mice for 16 weeks. ( h ) Correlations between serum LDL and 5-HT levels, IgE and 5-HT levels, LDL and NEFA levels, IgE and NEFA levels, and 5-HT and NEFA levels during EAT atrophy in HFD-fed mice. n=27. ( i ) Correlations between serum LDL and 5-HT levels, IgE and 5-HT levels, LDL and NEFA levels, IgE and NEFA levels, and 5-HT and NEFA levels in MASLD patients. n=50. ( j - l ) Differentiated 3T3-L1 adipocytes were treated with or without live BMMCs, BMMC lysates, LDL, LDL-activated BMMCs, DSCG- and LDL-treated BMMCs, or 5-HT. n=3/ea. ( j ) Representative images of oil-red O staining, media glycerol and NEFA levels in 3T3-L1 adipocytes. Scale bars: 50 µm. ( k/l ) Immunoblot analysis ( k ) and quantification of ( l ) ATGL, HSL and SIRT1 relative to β-actin, p-HSL(565) and p-HSL(563) relative to HSL, and p-AMPKα relative to AMPKα in 3T3-L1 adipocytes. Data are mean ± SEM. Pearson’s correlation test for h and i . P < 0.05 was considered significant correlation. Mann-Whitney U test for e . Kruskal-Wallis H test with Dunn’s post hoc adjustment for b-d , and f . Welch’s t-test for j and l . * p < 0.05, ** p < 0.01, *** p < 0.001. Different letters indicate statistically significant difference.

Article Snippet: To activate BMMCs, the cells were treated with 1 mg/mL LDL (Lee Biosolutions, Inc, Maryland Heights, MO Cat# 360-10).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Histone Deacetylase Assay, Saline, Double Staining, Staining, Western Blot, MANN-WHITNEY